Author: Douglas Orr (Page 7 of 8)

Check out this great Humboldt review by photosynthesis legend Susanne von Caemmerer, recently published in the Journal of Plant Physiology to commemorate the discovery of the dual nature of Rubisco some 50 years ago. This is a clear, very readable review of the ways the oxygenase side of Rubisco is fundamental to so much of our understanding of photosynthesis, and what its discovery allowed us to learn. Doug and Martin from the team have also contributed a short commentary, expanding a little on the engineering side of Rubisco and the ways engineering is trying to get around oxygenation.

Photosynthetic induction and its limitations.
New RIPE publication now out in PC&E:

Well done to Sam Taylor for leading a publication in Plant, Cell and Environment on variability in photosynthetic induction among closely related Brassica crops.
Alongside comparing these important species, Sam also developed enhanced gas exchange methods for more accurately determining photosynthetic induction and its limitations. Doug, Elizabete, and Steve were also co-authors on the study which is related to our work within the RIPE project to identify ways to improve photosynthetic induction in crops like cowpea, which are key to food security in sub-Saharan Africa.

Paper: https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.13862

Unraveling part of the ancient Rubisco puzzle:

Another publication with our friends at the Shih lab at UC Davis has recently been published in Nature Plants. This work looked at an ancient Rubisco identified from metagenomics data that lacks small subunits. This ancient form of the CO2-fixing enzyme represents a novel bacterial clade and is being termed a Form I’ Rubisco, composed of 8 large subunits (L8). Congrats to the team led by Doug Banda at UC Davis, with contributions also from UC Berkeley, the Lawrence Berkeley National Lab, and from the Lancaster Team Doug, Martin, and Elizabete.

Paper: https://www.nature.com/articles/s41477-020-00762-4

Great job by Bailey and a number of members of the team with the publication of work from Bailey’s MSc thesis in Agronomy journal.

Well deserved reward for all of Baileys hard work during her Masters research last year, where she investigated photosynthesis and water use efficiency in contrasting wheat cultivars at different developmental stages.

As part of her articles role as the first of a new Technical Innovations series at the Journal of Experimental Botany, and to bring attention to first authors through the ‘Celebrating First Authors’ videos, check out the short video by Cris to give a short context and description of her recently published work.

Video: Link

Big congrats to the Dr Gustaf Degen, who has successfully completed his PhD viva on his thesis research into Rubisco Activase in wheat.

He was supervised by Elizabete Carmo-Silva and Martin Parry.

He’s made a number of interesting findings, you can see some of these in a recent paper he led with Dawn and Elizabete, with more in the works.

Gustaf has been a great member of the team the last 3.5 years and has recently started a postdoc with Matt Johnson at the University of Sheffield.

Well done to Doug Orr and Elizabete Carmo-Silva who have recently contributed to an article characterizing Rubisco small subunit mutants published in the Journal of Experimental Botany.

This work was led by our friends in the McCormick lab at the University of Edinburgh with contributions from a number of collaborators. It was led by PK who has recently completed his PhD with Alistair on this work.

Congrats and well done to all.

Well done and great job by Cris Sales, Anabela Bernardes da Silva (Lisbon) and Elizabete Carmo-Silva who have recently published an article in the Journal of Experimental Botany and a related protocols piece.

This has been a big effort led by Cris to investigate the advantages and disadvantages of various methods of determining Rubisco activity and activation state, including ease of use, costs, and equipment requirements.

Cris has just recently started started a new postdoc working with Wanne Kromdijk at Cambridge.

The Lancaster Photosynthesis Team is excited to be recruiting two new Postdoctoral positions to contribute to the large multinational project RIPE: Realizing Increased Photosynthetic Efficiency for Sustainable Increases in Crop Yield. We are seeking two enthusiastic postdocs (Research Associates) to work in Plant Biochemistry and Plant Bioinformatics. These roles will contribute to the goal of improving Rubisco and its regulation in the legumes cowpea and soybean, in both fluctuating and steady-state conditions.

RIPE is funded by a sub-contract from the University of Illinois through the generous support of The Bill & Melinda Gates Foundation (BMGF), the UK Department for International Development (DFID), and the Foundation for Food and Agricultural Research (FFAR). The goal of this project is improve the productivity and sustainability of crops like cowpea, a vitally important crop for farmers in sub-Saharan Africa. As part of this project, you’ll have the opportunity to interact with a large team at Lancaster and within the broader project, to do world-leading research towards improving photosynthesis and improving the livelihoods of African farmers.

We are seeking people to work in the following areas (see links for full job description):
– Plant Biochemistry, with a focus on studying Rubisco, Rubisco activase and the interactions between these critical enzymes. This will involve both in vitro studies with recombinant proteins, and analysis of diverse germplasm of cowpea and soybean.

– Plant Bioinformatics, with a focus on understanding the genetic basis of variation in plant response to fluctuating light, with a focus on the regulation of Rubisco. This will include leading GWAS analyses, in addition to designing and executing experiments that take advantage of other NGS techniques (e.g. RNAseq).

All applications must be submitted through the Lancaster University Jobs portal located here.

For both positions, applications close on Saturday, September 7th.

Informal enquiries can be directed to the PIs of the project: Dr Elizabete Carmo-Silva e.carmosilva@lancaster.ac.uk , or Prof Martin Parry m.parry@lancaster.ac.uk.

The direct links are: Plant Biochemist: https://hr-jobs.lancs.ac.uk/Vacancy.aspx?ref=A2772

Plant Bioinformatician: https://hr-jobs.lancs.ac.uk/Vacancy.aspx?ref=A2771

Organising a conference: Plastid Preview 2018

By Rhiannon Page, Plastid Preview organising team member.

Plastid Preview is a small, friendly meeting for Early Career Researchers (ECRs) working in the area of chloroplast biology and I had been fortunate enough to attend twice, both in Edinburgh in 2016 and in Cambridge in 2017. So when I was given the opportunity to help organise the conference at Lancaster in 2018, I was super excited. However I was about to learn how much work goes into organising even a small event such as this! Luckily, other researchers in the group, Elizabete Carmo-Silva, Doug Orr and Mike Page, were also part of the organising team. The four of us sat down together almost a year before the meeting and started to make plans.

We set a date, wary to avoid a clash with other events in our field, and made a list of potential sponsors and supporters. Plastid Preview is traditionally free to attend for delegates so it was vital to find enough funding to cover all the costs. There was a bit of a rush to submit funding applications on time but thanks to the generosity of Phyconet, The Company of Biologists, Society for Experimental Biology, New Phytologist, Li-Cor, N8 AgriFood and Gatsby, the meeting could go ahead. It was an easy decision to use the Lancaster Conference Centre at Lancaster University as the venue and a quick meeting with their event organiser sorted the accommodation and conference dinner too. We designed a website to promote the conference and allow online registration and started inviting along participants.

As we eagerly watched the number of attendees increase, we started assembling a programme, abstract booklet and printing name badges. We wanted to make this year’s conference particularly useful to ECRs by trying to provide many opportunities for them to contribute actively to the meeting. We invited ECRs to chair the 6 seminar sessions. There were 20 talks and 18 posters supplemented with flash talks. Mary Williams (ASPB) and Mike Whitfield (New Phytologist) were asked to give talks on how ECRs can best promote themselves and their science, and included an excellent and fun interactive activity. We also arranged prizes for the Best Poster and Best Talk, and persuaded the PIs attending the conference to judge the competition.

When the day of the meeting finally arrived, we were a little tense but all our hard work paid off as the conference went smoothly and we could take part and enjoy proceedings along with all the delegates. I particularly enjoyed running the registration desk and welcoming everyone to Lancaster. This was a great opportunity to get to know all my fellow researchers and start building those all-important relationships. The conference dinner went well, providing a choice of meals and catering for all dietary requirements. As well as relaxing and networking, we managed to secure a willing volunteer to host next year’s Plastid Preview! We promoted the meeting on Twitter through the hashtag #plastidpreview2018, tweeting ourselves, with help from Ali Birkett, our department’s official tweeter, and encouraging participants to use the hashtag during the meeting. We reached over 100,000 impressions through 210 posts by 45 different users. People as far away as Australia, India, Chile and Brazil interacted with these tweets. Additionally, we requested that delegates provide feedback on the meeting and 40 people responded through a quick online survey. Please see the graphs below for a summary:

Following the conference there was still a surprising amount of work left to sort out! Reports needed writing for funders and supporters, travel claims arranged and finances put in order. Organising Plastid Preview 2018 was a lot of work but working with a team of great people made it a fun and exciting experience. I am immensely proud of what we all achieved and that Lancaster could contribute as host for this great series of meetings. We learnt during the meeting that Plastid Preview might have begun as long as 35 years ago! We hope it continues to be a useful meeting for ECRs and look forward to 2019’s edition in York later this year!

A new PhD position has just been advertised on the Lancaster Environment Centre website to join the photosynthesis team and work on Rubisco Activase from diverse plants.

This project is to work with two of our PIs: Elizabete Carmo-Silva and Marjorie Lundgren

If you or someone you know is interested, please head here for more information, the application process, and contact info.